July 21, 2014

Four Readings and an Open Science Argument

Here are some papers from my reading queue. Four readings on human culture, behavior, and evolution, and one feature (set of readings) on Open Science. 

Four Readings......

Here are four readings from the reading queue on human culture, behavior, and evolution. The picture below (only tangentially related to the first paper) is from [1].


The first paper [2] is on the genetic architecture of economic and political preferences. Using a SNPanalysis, the authors demonstrate that such traits have a polygenicarchitecture (e.g. many genes, small effect size for each). Studies that are underpowered (and no one really knows what the appropriate sample sizes should be) can potentially generate many false positive associations between genes and behavior.

The second paper [3] presents us with a premise that equates (or perhaps confounds) the psychophysiology of political ideologies with the roots of more general ideological bias. Are we really looking at "natural" differences between liberals and conservatives? Or does this simply demonstrate that high-profile social issues with already polar liberal and conservative positions [4] are undergirded by strong emotional responses? The standard evolutionary psychology explanation is a bit contrived as well. But it goes well with the previous article.


Crossmodal and cross-cultural comparisons, unite! In this study [5], people from several different cultures were asked to make both "congruent" and "incongruent" associations between smells and colors. The authors come to the conclusion that cultural context through experience has both statistical (covariance) and semantic (linguistic) components.

The fourth article [6] is a gateway article to several recent studies in the area of neuroplasticity. The gateway leads to the work being done in the laboratory of Michael Stryker [7]. Learn about the "neural volume control knob" and much, much more.


.....And An Open Science Argument


Here are some additional readings on networking and open science from my reading queue. The first is a paper on the life-cycle of a preprint on the arXiv [8] The top image is Figure 2 in the paper. The other two readings advocate for the use of open access protocols and social media to disseminate research [9] and counter cultural biases towards keeping research behind laboratory doors [10].


NOTES:

[2] Benjamin, D.J. et.al    The genetic architecture of economic and political preferences. PNAS, 10:1073/ pnas.1120666109 (2014).


[4] Related to this is the concept of the news filter bubble. One recent paper on this phenomenon: Koutra, D., Bennett, P., and Horvitz, E.   Events and Controversies: influences of a shocking news event on information seeking. arXiv, 1405.1486 (2014).

[5] Ren et.al   Cross-Cultural Color-Odor Associations. PLoS One, 9(7), e101651 (2014).

[6] Stix, G.   Neuroplasticity: new clues to just how much the adult brain can change. Scientific American blog, July 14 (2014).

[7] Two notable publications:
a) Fu, Y., Tucciarone, J.M., Espinosa, S., Sheng, N., Darcy, D.P., Nicoll, R.A., Huang, J., and Stryker, M.P.   A Cortical Circuit for Gain Control by Behavioral State. Cell, 156, 1139–1152 (2014).

b) Niell, C.M. and Stryker, M.P.   Modulation of Visual Responses by Behavioral State in Mouse Visual Cortex. Neuron, 65, 472-479 (2010).

[8] Shuai, X., Pepe, A., and Bollen, J.   How the Scientific Community Reacts to Newly Submitted Preprints: Article Downloads, Twitter Mentions, and Citations. PLoS One, 7(11), e47523 (2012).

[9] Allen , E.   “All research should be OA”. We agree! ScienceOpen blog, July 14 (2014).

[10] Konkiel, S.   How to become an academic networking pro on LinkedIn. ImpactStory blog, April 24 (2014).

July 15, 2014

Free Artificial Life (XIV edition) and Other Evolution Readings


Here is some Artificial Life for your summer reading. The Proceedings of Artificial Life XIV (complement to the conference at the end of this month in New York City) is a peer-reviewed venue for papers covering the following topics: evolutionary dynamics, soft robots, agent behavior, collective behaviors, social dynamics and evolution, and cellular automata/ self-organizing systems.


And continuing in the spirit of evolutionary scholarship, here are a few more readings from the queue in the last few months. I would call it the "summer of evolution", but every summer is the season of evolution.

I. Reticulating Clades (not Splines) and the Three Domains


Is it possible that the three domains of life are not monophyletic? A new study in Nature Reviews Microbiology [1] using cutting-edge phylogenetic methods suggests that they are not [2]. As an alternative, they suggest the ring of life hypothesis summarized above, with reticulations between each domain's clade Perhaps we are all not just Eukaryotic, and in a manner that does not directly involve symbiosis.


II. Saturday Morning Skepticism


Saturday Morning Breakfast Cereal meets potential evolutionary mechanisms. This comic from Zach Weinersmith is a joke about Horizonal Gene Transfer (HGT). Which got me to thinking: how over-hyped is HGT? The following article [3] and blog posts [4, 5] should give you some idea, as well as to the true scope of the phenomenon. Short answer: selective in Prokaryotes, nearly non-existent in Eukaryotes, and the enabler of salterns, sushi, worms and aphids [3, 6].


NOTES:
[1] McInerney, J.O., O’Connell, M.J., and Pisani, D.   The hybrid nature of the Eukaryota and a consilient view of life on Earth. Nature Reviews Microbiology, doi:10.1038/nrmicro3271 (2014).

[2] McInerney, J.   The three domains of life hypothesis has been falsified. Bioinformatics and Molecular Evolution Unit blog (2014).

[3] Zhaxybayeva, O. and Doolittle, W.F.   Lateral Gene Transfer. Current Biology, 21(7), R242-R246 (2011).

[4] Coyne, J.   How important is lateral Gene Transfer? Why Evolution is True blog, April 13 (2011).

[5] Kuchner, T.   Horizontal Gene Transfer Takes a Turn: Expansins from Plants to their Bacterial and Eukaryotic Parasites. Molecular Evolution Forum blog, March 14 (2014).

[6] Boto, L.   Horizontal gene transfer in the acquisition of novel traits by metazoans. Proceedings of the Royal Society B, 281, 20132450 (2014).

The diverse places where HGT occurs. COURTESY: Figure 1 in [3].




July 8, 2014

Contributions to the bioRxiv, Summer 2014

I have been busy finishing up some work done in the Cellular Reprogramming Lab between 2010 and 2013. These two papers were submitted to and rejected as a single paper from PLoS One (after two rounds of revision). They were subsequently split them into their wet-lab molecular biology (written as an extended protocol) and computational components (written as a more conventional manuscript) for publication on bioRxiv.



The first paper (wet-lab molecular biology) is called "Using Polysome Isolation with Mechanism Alteration to Uncover Transcriptional and Translational Dynamics in Key Genes", in which we explore the world of mRNA regulation during adaptive cellular processes. The first part of the title (polysome isolation) involves harvesting mRNA from the polysome (translation-related mRNA). Harvesting this in tandem with mRNA associated with transcription provide us with a direct comparison between the transcriptome (TST) and translatome (TLT).


The second part of the title involves administering drug treatments to fibroblast populations which have systematic effects on transcription and protein production. These treatments are called "mechanism alteration" because they mimic changes that occur in a dying or transforming cell.


The third part of the title involves looking at transcriptional and translational dynamics for key genes. One criticism of the combined paper involved the use of candidate genes instead of high-throughput data. High-throughput data is great if one can afford it. On the other hand, large datasets can leave you with more questions than answers, which might be particularly true of this work. 


These figures demonstrate the analysis of experiments which validate the polysome recovery technique and the effects of drug treatments (mechanism disruption) on both transcriptome- and translatome- related mRNA (TST and TLT, respectively).

The second paper (computational) is called "Modeling Cellular Information Processing Using a Dynamical Approximation of Cellular mRNA". There is also a Github repository that contains associated Matlab code and simulations. Work from the first and second papers have been presented previous to their bioRxiv release, notably at the Stem Cell and Regenerative Medicine Conference held on the Oakland University (MI) campus in 2012.


We will go through this title in backwards order this time. The last part (cellular mRNA) refers to its connection to the first paper. Gene expression measured at both the transcriptome (TST) and translatome (TLT) will be used to model the cell's general response to mechanism alteration. In this case, an assumption is made: fluctuations of both mRNA fractions and at multiple points in time represents a regulatory process. 

Thus, a first-order feedback model can be constructed, with a simplified set of feedforward, feedback, and decay components. While there are a multitude of mRNA decay pathways and processing functions, this model focuses on a much simpler abstraction: the path from DNA to protein with single sources of decay and feedback. Each fraction of mRNA can be represented as a point process controlled by inputs and outputs.


The middle part of the title (dynamical approximation) then refers to the simulation of mRNA dynamics using the model, its components, and biological data. The idea is to approximate meaningful trends at certain points in a biological process, which is expected to differ by gene and by model component. This is where the proof-of-concept nature of this paper is most evident. 

While a somewhat contrived means to approximate a complex biological process is used in both papers, the original application was to be for understanding the early stages of cellular reprogramming. However, it proved to be exceedingly difficult to go from iPS cultures to meaningful computational inference.

An example of the first-order feedback model. A: a graphical example of the model components. B: an example of activity among the components over time.

Finally, the first part of the title (modeling cellular information processing) is based on the interpretation of the model output. the notion of cellular information processing treats the regulation of mRNA as an information processing problem. That is, you have an input, a process, and an output. systematic noise can also be added to the model, depending on the application. The process itself (mRNA processing from DNA transcription to RNA translation) is a transformation of information. 

When a cell is challenged by an environmental stimulus or the need to change phenotype, information provided by mRNA can be operated upon in a number of ways (linear responses, accumulation, delayed responses). Three information processing principles are used to interpret phenomena such as linear decay, the sequestration of mRNA at either the TST or TLT, and the differential response among individual genes.


Finally, I must point out how cool the altimetric support is on the bioRxiv. Here is a screen shot showing the number of tweets, abstract views, and .pdf downloads for the wet-lab paper:


Almost as functional as the analytics data on Blogger (which is not saying much, but for a formal publication venue, it's pretty impressive). The people at Cold Spring Harbor Lab have done a good job on this, and are ahead of the people at arXiv on this. As it turns out, however, the arXiv has a principled policy on this. Whether viewership stats are a sign of vanity and worthy of scare quotes is another matter.


July 6, 2014

Carnivals and Toy Models

Two items of business (blog carnival and new paper announcements) in this post, neither of which involve carnival toys or any variant thereof. But keep reading anyway.

Nothing to see here. Move along.

Carnival of Evolution #73:

 
Time for another Carnival of Evolution. In this edition (#73) hosted by Pleiotropy blog, the theme is a tournament-style presentation based on the ongoing World Cup. This month’s Synthetic Daisies submission lost a close one early, and you will have to read CoE 73 to know who won the whole thing. Thanks go to BjornOstman for all of his work and the clever theme. Have an evolution-related blog post that needs publicizing? Please submit it to the CoE Facebook page.



Alicea, B. and Gordon, R.   Toy models for Macroevolutionary Patterns and Trends, Biosystems, 122, 25-37.


I invite you to take a look at a new paper by myself and Richard Gordon called "Toy Models for Macroevolutionary Patterns and Trends", out now in the journal Biosystems [1]. This will eventually be part of a special issue called "Patterns of Evolution". There is also a Github repository, which will house examples of toy models and other supplemental information. The paper reviews and/or describes 13 toy models, some pre-existing and others brand new examples. Toy models are representations that are intentionally oversimplified, used to approximate overarching trends while at the same time being sensitive to evolutionary context.

 The coupled avalanche model, an example of a macroevolutionary toy model.

We introduce 13 different toy models that cover a range of macroevolutionary phenomena such as the generation of diversity, the representation of lineages, and nonlinear evolutionary changes. There is also an undercurrent of meta-theory and why that is important to evolutionary theory-building.

The paper also provides examples of application domains, such as Artificial Life simulations and the analysis of high-throughput data. Toy models can also be used in tandem to approximate difficult evolutionary problems. While I do not want to give away too much of the details, I will say that the paper should prove useful to hard-core biologists, evolutionary modelers, bioinformaticians, and philosophers of science alike.

[1] the link provided might lead you beyond the Great Wall of Rentier. If you need a copy, e-mail me.


June 30, 2014

Thought (Memetic) Soup, June edition

Happy middle of summer (in the Northern hemisphere, anyways)! Here are some humorous and puzzling items from my leisure time, cross-posted to Tumbld Thoughts. Also an update on Orthogonal Research, which is turning into quite a productive endeavor. This also marks the return of the Thought Soup series. Bemusement and incredulity abound. 

I. Technological Bemusement (for better and for worse)


Miguel Nicolelis (Neuroscientist of BMI fame) is demoing an EEG-controlled exoskeleton at the World Cup [1]. The exoskeleton is able to engage in soccer-related movements, but is controlled by a human brain. Read the Science News interview for more. And here is the outcome [2], courtesy of Neurogadget.




Contrary to the popular trough of disillusionment, Google Glass is a huge development in the world of Augmented Reality. Soon we will all be wearing glass-mounted displays, even if they are not made by Google. Google is apparently very bad at marketing, but that may not be the whole story [3]. Just know that violent responses to so-called "glassholes" is not entirely new.


25 years ago this month: Star Trek V opens. See William Shatner direct a film. Then see William Shatner rock-climb (poorly) and question God. If God is at the center of our galaxy, then are there gods at the center of all of the other galaxies? And if a god created the Big Bang (as some people claim), then is this God merely a middle manager? These are the types of questions audiences should have been asking, but it was 1989 and we were all fascinated with Spock's levitation boots.


II. Speaking Fee Incredulity


Speaking fee incredulity, courtesy of CREMA [4]. The graph is a sampling of economists on the lecture circuit: the x-axis is their relative internet ubiquity, and the y-axis is their minimum speaker's fee. Notice the red arrow and how it points to a cohort that includes Myron Scholes, Dan Kahneman, and Ben Stein. Funny how the world works sometimes.

III. Spam and Pointless Political Resistance Incredulity

I quit, I give up, Nothing's good enough for anybody else, It seems  -- Circle, Edie Brickell.
The chorus of this song [5] seems to summarize the Democratic Party's 2014 grass-roots fundraising strategy against the conservative Super-PAC fundraising [6]. Do you approve of this message? There are some people (progressive-minded bloggers, no less) who do not [7]. I don't think the Queen (a tory who does not have to worry about being elected) is amused, either. 


Of course, it's only a matter of time before democracy-as-market-economics [8] implodes. Perhaps we are witnessing that implosion right now. In the meantime, enjoy this picture of a jihadist stroking his cat. No, it's not a dirty limerick -- it's some form of PoMo resistance. Is this the height of absurdity, or Dr. Evil, martyrdom edition? Big money and bad religion, it's all highly-offensive performance art to me.



IV. Lack-of-funding Incredulity


My slouch towards intangible forms of enterprise continues. The Orthogonal Research activity report for the second quarter (Q2) of the calendar year (not financial) is now available. Busy quarter, but still without funding (although parties interested in changing that can contact me).

Is placing a value on research necessary but not sufficient? Here's one humorous take. COURTESY: PhD Comics.


NOTES:
[1] Servick, K.   Kickoff looms for demo of brain-controlled machine. Science News, 344(6188), 1069-1070 (2014).

[2] Paraplegic Man In Mind-Controlled Robotic Suit Kicks Off World Cup 2014. Neurogadget, June 13 (2014) AND Atkins, H.   Human In Robotic Exoskeleton To Kick Off The World Cup. Popular Science, June 6 (2014).

[3] Edwards, J.   Google glass is going to be huge, and its critics are wrong. Business Insider, June 9 (2014).



[5] Circle, Edie Brickell and the New Bohemians. YouTube video (1988).

[6] Dear Democrats, please stop spamming me for donations. Weasel Zippers blog, February 28 (2013).

[7] Atrios.   From Bean to Cup, You Fuck Up. Eschaton blog, May 27 (2014) AND Myers, P.Z. Democrats: you suck. Pharyngula blog, May 30 (2014).

[8] Avalon, J. and Keller, M.   The Super PAC Economy. Daily Beast, September 18 (2012) AND Aronsen, G.   Are Super PACs Overhyped? Mother Jones, September 28 (2012).

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